DNA-epoxyceliulose to sequence-specific DNA-binding proteins
نویسنده
چکیده
Phage A DNA was covalently coupled to epoxy-activated cellulose to form a stable DNA-cellulose matrix for affinity chromatography of sequence-specific DNAbinding proteins. The accessibility of three specific six-base sequences, GGATCC (BamHI), GAATTC (EcoRI) and AAGCTT (HindIII) was studied quantitatively and qualitatively by restriction analysis followed by labelling of their recessed ends. All sites are randomly accessible. The site accessibility is variable, BamHI> HindlIl > EcoRI, and within the range 20-100% depending on base composition and internal structure of the sequence. DNA-epoxycellulose, because of its high efficiency of coupling, capacity, stability and accesslbility, can be of great help in the isolation and characterization of sequence-specific DNA-binding proteins.
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تاریخ انتشار 2005